Microbial and phenotypic definition of rats and mice. Shell surface irregularly longitudinally ribbed, without conspicuous pits or depressions, almost smooth. Antibody response to infection with mouse pinworm syphacia obvelata was studied in akrj mice using an enzymelinked immunosorbent assay elisa. A new hope to induction of intestinal immunological tolerance in c57bl6 mice niloofar taghipour, 1, 2 nariman mosaffa, 3, mohammad rostaminejad, 2 mohamad mohsen homayoni, 4 esmaeil mortaz, 3, 5 hamid asadzadeh aghdaei, 6, and mohammad reza zali 2. To differentiate the morphologically similar pinworms of the common laboratory rodents, such as syphacia obvelata and syphacia muris, we amplified and sequenced the region spanning the internal transcribed spacer 1 its1, 5. Pinworms of the order oxyurina syphacia muris, syphacia obvelata, and aspiculuris tetraptera are commonly found in laboratory animals. Pdf data on the frequency, distribution and mean intensity of the helminth fauna. Patterns of infection with the nematodes syphacia obvelata. Syphacia muris is the most common oxyurid of the rat liang et al.
Instead each of two moults were observed outside the egg up to 24 and 40 hours after infection, and tem studies suggest a third moult occurs up to 64 hours. Syphacia obvelata deposits eggs in the perianal region while aspiculuris tetraptera releases eggs in the colon which then. Syphacia obvelata, the common mouse pinworm, is a ubiquitous parasite of wild and. Pdf patterns of infection with the nematodes syphacia. Antihelminic activity of carvacrol, thymol, cinnamaldehyde and pcymen against the freeliving nematode caenorhabditis elegans and rat pinworm syphacia muris s. Pdf patterns of infection with the nematodes syphacia obvelata.
The lifecycle of syphacia muris is described in primary infections of wistar rats. Pdf different treatment regimen for eradication of pinworm. Syphacia obvelata deposits eggs in the perianal region while aspiculuris tetraptera releases eggs in the colon which then are passed in fecal pellets. University of cape town,faculty of health sciences,division of immunology, 2006 cited yyyy month dd. Aspiculuris tetraptera larvae live in the crypts of lieberkuhn in the proximal colon, after hatching in the cecum. The prevalence of syphacia muris was highest in mongolian gerbils where up to 75. Evaluation of coinfection with pinworms aspiculuris. Syphacia obvelata is a pinworm that belongs to the order oxyurina and lives in cecum and anterior colon of mice.
The prepatent periods of syphacia obvelata and syphacia muris are 1115 and 811 days, respectively. In this study, we show the consequence of an outbreak in a transgenic barrier facility of infection by syphacia obvelata, a murine pinworm gastrointestinal nematode. Unfortunately, this book cant be printed from the openbook. Dec, 2019 syphacia obvelata is a pinworm that belongs to the order oxyurina and lives in cecum and anterior colon of mice. Oxyuroidea in the laboratory rat volume 60 issue 1 j. Infection with syphacia obvelata pinworm induces protective. Understanding and treating pinworm infection in rodent. Text is available under the creative commons attributionsharealike license. Infection with syphacia obvelata pinworm infection and immunity. The main aim of this study was to investigate the immunological patterns of s. Mice were orally infected with pinworm eggs and their sera checked for antibodies. Immunomodulatory effect of syphacia obvelata in treatment.
Summarythiabendazole incorporated in the diet at a rate of 01%, fed to all animals over a period of 3 months, successfully eliminated the parasite from the colony. Patterns of infection with the nematodes syphacia obvelata and. Syphacia oryzomyos is a nematode that infects the marsh rice rat oryzomys palustris in florida. The video shows how the eggs are moving in the body. Immunomodulatory effect of syphacia obvelata in treatment of. Eggs of syphacia species are infective within 520 hours. To determine optimal detection methods, swiss webster mice were exposed to syphacia obvelata so. The oxyurid nematodes syphacia obvelata and aspiculuris tetraptera presented overall frequencies of 91.
Studies on the life cycle of syphacia obvelata, a common nematode parasite of rats. This oxyurid genus is distributed worldwide and has zoonotic potential 23, and a human case infested with syphacia obvelata has been reported in the philippines 16. Kamfai chan, life cycle studies on the nematode syphacia obvelata, american journal of epidemiology, volume 56, issue 1. Efficacy of ivermectin against syphacia obvelata nematoda in mice volume 59 issue 3 d. Syphacia medical definition merriamwebster medical dictionary. Syphacia obvelata has been reported to be zoonotic 6 as well as more common in immunocompromised young males.
Syphacia syphacia wikispecies, free species directory. For example, altered growth of certain strains of rats and mice has been linked to syphacia muris infestation, 27, 39 and impaired electrolyte transport in spontaneously hypertensive s. Oxyuriasis, or pinworm infection, is very common in mouse and rat colonies with the two species most often diagnosed in mice being. The nematodes syphacia obvelata rudolphi, 1802 seurat, 1916 and aspiculuris tetraptera nitzsch, 1821 schulz, 1924 were recovered from the investigated mice. The rat, gerbil, and hamster are also occasionally infected. The direct transmission of these gastrointestinal gi nematodes by contaminated food, water, and bedding results in continual reexposure to the parasite, making. The eggs of syphacia obvelata are very light and have been shown to aerosolize, resulting in widespread exposure. A mouse oxyurid, syphacia obvelata, as a parasite of man is an article from the journal of parasitology, volume 6.
Still more striking are the differences between the eggs of the two species, those of syphacia obvelata figs. Pinworms of the order oxyurina syphacia muris, syphacia obvelata. Syphacia species adult females migrate from the cecum through the colon to the rectum, and deposit their eggs in a bolus on the perianal area. Nov 12, 2016 i found lots of these inside wild mice intestines. All had better detection rates than sheather sugar flotation 50%. An oral combination of piperazine and ivermectin was used over a 6week period for treating three different colonies of mice or rats infested with syphacia obvelata, syphacia muris or aspiculuris tetraptera. Animals on study were maintained in an isolated quarantine area apart from the main colonies. This is opposed to a facultative parasite, which can act as a parasite but does not rely on its host to continue its lifecycle.
Life cycle studies on the nematode syphacia obvelata 1 journals. The direct transmission of these gastrointestinal gi nematodes by contaminated food, water, and bedding results in continual reexposure to the parasite, making the control of pinworms in animal holdings quite difficult. If an obligate parasite cannot obtain a host it will fail to reproduce. The first page of the pdf of this article appears above.
This page was last edited on 19 october 2019, at 17. Syphacia obvelata infection was diagnosed in mice during routine health monitoring of rodent colony at animal facility, ilsniser, bhubaneswar. Studies on the life cycle of syphacia obvelata, a common. The females migrate to the anus and lay their adhesivecoated eggs directly on the perianal skin. Syphacia muris, syphacia obvelata and aspiculuris tetraptera. Eradication of the pinworm syphacia obvelata from an animal. Syphacia definition of syphacia by medical dictionary. The results of some studies using this nematode suggest it can modulate autoimmune. Oxyuriasis pinworms division of animal resources university of illinois, urbana background. Dorde marjanovic 1, danica bogunovic 2, mirjana milovanovic 1, darko marinkovic 3, nemanja zdravkovic 4, vladimir magas 5 and m. A series of coumarinbased trisubstituted methanes trsms having uracil scaffold was synthesised employing a green, chromatographyfree, and a highly efficient sonochemical multicomponent reaction of diverse aldehydes with 1,3dimethyl6aminouracil and 4hydroxycoumarin in the presence of a catalytic amount of dabco at room temperature and tested their. Syphacia obvelata is the common cecal pinworm of mice, and syphacia muris, of rats.
Specific pathogen free spf list for xenopus laevis agent syndrome detection aeromonas hydrophila septicemic syndrome culture. Syphacia obvelata deposits eggs in the perianal region while aspiculuris tetraptera releases. Syphacia obvelata is more frequently found in mice, hamsters, and gerbils but is also occasionally found in the rat, especially when housed in the same room with infested mice. We compared the following methods at days 0, 30, and 90, beginning 21 or 28 d after so and at exposure, respectively. Obligate parasites have evolved a variety of parasitic. Characterization of rdna sequences from syphacia obvelata. Syphacia muris enteric helminths direct exam of cecumfeces. A similar species of syphacia has been recorded from the rice rats oligoryzomys fulvescens and handleyomys melanotis in san luis potosi, but because only females were found, this worm could not be identified to species. Pinworms of the order oxyurina syphacia muris, syphacia obvelata, and aspiculuris tetraptera are. The latter highlights the importance of maintaining mice under pinwormfree conditions.
Summarythiabendazole incorporated in the diet at a rate of 01%, fed to all animals over a period of 3 months, successfully eliminated the parasite. The oxyurid nematodes syphacia obvelata and aspiculuris tetraptera presented. A new hope to induction of intestinal immunological tolerance in c57bl6 mice. Syphacia numbers diminish with increasing age of the host wescott, 1982 athymic nunu mice have increased susceptibility jacobson and reed, 1974 mastomys coucha is more susceptible than the balbc mouse higgins et al. Efficacy of ivermectin against syphacia obvelata nematoda. Different treatment regimen for eradication of pinworm. This parasitological study was carried out during the period of march 2014february 2015 to investigate the helminth parasites infecting the laboratory mice mus musculus in the animal house at cairo university, egypt. Eradication of the pinworm syphacia obvelata from an. The most important criteria for the introduction of other nematodes is recognition and availability of them. Effective eradication of pinworms syphacia muris, syphacia. Syphacia obvelata is a pinworm nematode parasite infecting man and laboratory animals in high abundance. Syphacia medical definition merriamwebster medical.
All these worms deposit eggs during periods of their hosts inactivity presumably to avoid being lost otherwise. Mongolian gerbils meriones unguiculatus naturally infected with the nematode dentostomella translucida and mice mus musculus that were either pinwormfree or harboring either or both aspiculuris tetraptera and syphacia abvelata were crossinfected experimentally with these nematode species. These nematodes belong to the order oxyurina syphacia obvelata and syphacia muris and are colonized in large and small intestines of mice and rats. Infections with pinworms are common in rodent animal facilities. Patterns of infection with the nematodes syphacia obvelata and aspiculuris tetraptera in conventionally maintained laboratory mice. Camille harris, in manual of exotic pet practice, 2009. Rats were also free of ectoparasites and endoparasites other than syphacia muris on inhouse screening tests. Rodent pinworm fact sheet vice president for research.
Uninfected mice kept under specificpathogenfree conditions in a different animal room were used. Syphacia obvelata and aspiculuris tetraptera are nematodes commonly found in mice and other rodents. Evaluation of traditional and contemporary methods for. For full access to this pdf, sign in to an existing account, or purchase an annual subscription. Investigation the immunological response elicited to the. Understanding and treating pinworm infection in rodent colonies. Characterization of rat pinworm syphacia muris epidemiology. Syphacia obvelata primerdesign ltdtm 150 tests genesig standard kit for general laboratory and research use only quantification of syphacia obvelata genomes. Syphacia obvelata an overview sciencedirect topics. Investigation the immunological response elicited to the gastrointestinal nematode pinworm syphacia obvelata. At the same time mice were isolated and grouped n18 to compare. The rat pinworm, syphacia muris, may also infect mice, and rats may also be infected with mouse species. Oxyurina syphacia obvelata, syphacia muris, aspiculuris.1265 639 59 378 1337 478 1435 319 727 528 1396 1375 144 45 842 1277 1452 1125 1416 340 991 966 1076 1336 1375 1145 974 25 186 1240 1325 1195 252 1439